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Internet Electronic Journal of Molecular Design - IEJMD, ISSN 1538-6414, CODEN IEJMAT
| ABSTRACT - Internet Electron. J. Mol. Des. February 2005, Volume 4, Number 2, 106-123 |
Sweet and Taste Modifying Proteins - Comparative Modeling and
Docking Studies of Curculin, Mabinlin, Miraculin with the T1R2-T1R3 Receptor
Ravi Kant, Mohan Babu Rajasekaran, and Ramakumar Suryanarayanarao
Internet Electron. J. Mol. Des. 2005, 4, 106-123
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Abstract:
Sweet and taste-modifying proteins and their response to the
T1R2-T1R3 G-protein coupled sweet taste receptor is still a topic
of discussion. In search of low caloric natural sweeteners for
diabetic patients, comparative modeling of curculin, mabinlin,
miraculin and T1R2-T1R3 receptor were performed as no X-ray
or NMR structure was available for any one of them.
Comparative modeling and docking was done using SwissModel
server and GRAMM software, respectively. Docking of proteins
with the sweet taste receptor was followed by analysis of
protein-protein complexes with various parameters and was found nearly
stable, except in the case of mabinlin. Based on the results, we
propose that sweet and taste modifying proteins curculin and
miraculin will bind with sweet human taste receptor T1R2-T1R3
and will perform biological activity.
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